Fmoc-Gly-Wang Resin

Fmoc-Gly-Wang Resin
Nα-Fmoc-glycine esterified to Wang (4-alkoxybenzyl alcohol) resin; 100–200 mesh, 0.3–0.6 mmol/g

Wang resin preloaded with Fmoc-Gly-OH, for making peptide acids with a C-terminal glycine by Fmoc SPPS. Remove the Fmoc group with 20% piperidine in DMF and build the chain as usual; final cleavage with 95% TFA and scavengers releases the peptide acid and removes the side-chain protecting groups. The loading for each lot is on its certificate of analysis.

Glycine is achiral, so the C-terminal residue cannot epimerize. Diketopiperazine formation is more of a risk: at the dipeptide stage the free amine can attack the ester bond to the resin, especially when glycine or proline is one of the first two residues. Couple the third residue promptly after the second Fmoc removal, or start on 2-chlorotrityl resin for such sequences.

Applications

• Peptide acids with a C-terminal glycine
• Manual, microwave and automated synthesis
• Peptide libraries and parallel synthesis

Storage
Store refrigerated (2–8 °C) and dry, in a tightly closed container.

Published research using P3 BioSystems material

• Hernandez ET et al. 2,2'-Bipyridine and hydrazide containing peptides for cyclization and complex quaternary structural control. New Journal of Chemistry 2018. doi:10.1039/c8nj00184g

References

1. Wang S. p-Alkoxybenzyl Alcohol Resin and p-Alkoxybenzyloxycarbonylhydrazide Resin for Solid Phase Synthesis of Protected Peptide Fragments. Journal of the American Chemical Society 1973, 95, 1328–1333. doi:10.1021/ja00785a602
2. Fields GB et al. Solid phase peptide synthesis utilizing 9‐fluorenylmethoxycarbonyl amino acids. International Journal of Peptide and Protein Research 1990, 35, 161–214. doi:10.1111/j.1399-3011.1990.tb00939.x

For research use only. Not for human or veterinary use.